论文期刊

Cytocompatibility and antibacterial activity of nanostructured H2Ti5O11•H2O outlayered Zn-doped TiO2 coatings on Ti for application in percutaneous implants




作者: Lan Zhang,Juan Zhang, Yong Han
发表/完成日期: 2017-10-03
期刊名称: Scientific Reports
期卷:
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论文简介
In order to endow Ti with enhanced skin-integration and antibacterial activity simultaneously, novel coatings comprising an outer layer of nanoplate/nanorod/nanofibre H2Ti5O11•H2O (HTO) and an inner layer of microporous Zn-doped TiO2 were fabricated by a hybrid process of micro arc oxidation (MAO) and hydrothermal treatment (HT). The microstructure, adhesion strength, hydrophilicity and micro-roughness of MAOed coating after HT for 1, 4, 6 and 48 h were examined; the adhesion, proliferation, phenotype, differentiation and extracellular collagen secretion of fibroblasts, as well as the response of gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli), were also investigated. During HT, TiO2 was attacked by OH- from HT solution, forming HTiO3-. HTiO3- reacted with TiO2 and H2O, resulting in the nucleation of HTO. When HT process prolonged, HTO nuclei grew to nanoplates, nanorods and nanofibres. Simultaneously, the orientations of nanorods/nanofibres also changed, appearing to be quasi-vertical and parallel to the underlying substrate, respectively, corresponding with the slightly widening of inter spacing. The HTed coatings adhered firmly to Ti substrates and exhibited super-hydrophilic. Compared to Ti, the adhesion and proliferation of fibroblasts were enhanced on the as-MAOed TiO2 and HTed coatings, especially on HT4h. The phenotype, differentiation and extracellular collagen secretion were obviously accelerated on vertical nanorods on HT4h due to its proper interspace (e.g. 63 nm). HTed coatings showed antibacterial properties in similar degree with as-MAOed TiO2. The antibacterial activity of as-MAOed TiO2 were ascribed to the ROS and zinc ion from the coating. Whereas, the antibacterial activity of HTO should be ascribed to its nano-topography.